德國(guó)Emfret公司專(zhuān)注心血管和血液系統(tǒng)研究用抗體研發(fā)和生產(chǎn)，供應(yīng)的抗體包括清除血液中血小板、流式細(xì)胞術(shù)鑒定分析小鼠血小板表面糖蛋白，emfret公司的抗體不僅適用于流失分析、WB檢測(cè)，還可用于免疫組化/免疫熒光及免疫共沉淀檢測(cè)。

背景資料：

This antibody preparation contains a mixture of purified rat monoclonal antibodies directed against mouse GPIb (CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent platelet depletion in mice1,2 . Platelet depletion is frequently used to study the general role of platelets in (patho-) physiology3 .

保存條件：

The antibodies were purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography.

Stable for six months from date of shipment when stored at 4°C.

KEEP STERILE, the preparation contains no preservative. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.

應(yīng)用：

This preparation is optimized to reduce the platelet count by >95% within 60 minutes after intravenous injection of 2 µg/g bodyweight. It is recommended to inject the antibodies in a volume of 100-200 µl (diluted with sterile PBS).

同型對(duì)照（抗體來(lái)源）： Rat IgG (Wistar)

緩沖液： 0.5 mg purified immunoglobulins in PBS, 0.2% (w/v) BSA, sterile filtered

濃度： 0.5 mg/ml

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